Journal: Pulmonary Circulation
Article Title: Increased plasma expression of a disintegrin and metalloproteinase with thrombospondin motifs like 4 in patients with idiopathic pulmonary arterial hypertension and chronic thromboembolic pulmonary hypertension
doi: 10.1002/pul2.12267
Figure Lengend Snippet: ADAMTSL4 expression in PASMCs. The protein expression of ADAMTSL4 in PDGF‐BB‐treated PASMCs were detected using Western blot. * p < 0.05 versus the control group. ADAMTSL4, a disintegrin and metalloproteinase with thrombospondin motifs like 4; IPAH, idiopathic pulmonary arterial hypertension; PASMCs, pulmonary artery smooth muscle cells; PDGF, platelet‐derived growth factor.
Article Snippet: Primary rat pulmonary arterial smooth muscle cells (PASMCs) were isolated from healthy Sprague‐Dawley rats (6–8 weeks, 180–200 g) and cultured in Dulbecco's modified Eagle's medium/nutrient mixture F‐12 containing 20% fetal bovine serum and 1% penicillin and streptomycin.
Techniques: Expressing, Western Blot, Control, Derivative Assay